8SJV

[4T24] Self-assembling left-handed tensegrity triangle with 24 interjunction base pairs and R3 symmetry

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-13
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.00000
Spacegroup name	H 3
Unit cell lengths	225.409, 225.409, 88.324
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.750 - 8.590
R-factor	0.1825
Rwork	0.179
R-free	0.28030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w6w
RMSD bond length	0.004
RMSD bond angle	0.664
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	80.470	80.470	9.512
High resolution limit [Å]	8.265	16.998	8.265
Number of reflections	1125	188	189
<I/σ(I)>	9.5	28.9
Completeness [%]	79.8	99.5
Redundancy	11	10.8	10.2
CC(1/2)	0.999	0.999	0.370

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	293	100 mM MOPS, 1.25 M magnesium sulfate