8SJU

[4T17] Self-assembling right-handed four-turn tensegrity triangle with 17 interjunction base pairs and R3 symmetry

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-24
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.00743
Spacegroup name	H 3
Unit cell lengths	224.532, 224.532, 126.522
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.170 - 7.140
R-factor	0.1126
Rwork	0.111
R-free	0.17740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w6w
RMSD bond length	0.004
RMSD bond angle	0.609
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	77.092	77.092	7.690
High resolution limit [Å]	6.988	18.847	6.988
Number of reflections	2501	190	189
<I/σ(I)>	8.7	20
Completeness [%]	84.6	98.4
Redundancy	10.7	10.5	11.1
CC(1/2)	0.995	0.995	0.276

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	293	100 mM MOPS, 1.25 M magnesium sulfate