8SJS
[3T18] Self-assembling right-handed tensegrity triangle with 18 interjunction base pairs and P63 symmetry
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-16 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 63 |
| Unit cell lengths | 194.203, 194.203, 55.434 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.780 - 6.310 |
| R-factor | 0.1102 |
| Rwork | 0.110 |
| R-free | 0.11910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5w6w |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.492 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 97.102 | 97.102 | 7.018 |
| High resolution limit [Å] | 6.307 | 15.535 | 6.307 |
| Number of reflections | 2039 | 204 | 207 |
| <I/σ(I)> | 12.6 | ||
| Completeness [%] | 86.1 | ||
| Redundancy | 19.4 | ||
| CC(1/2) | 1.000 | 0.999 | 0.305 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 100 mM MOPS, 1.25 M magnesium sulfate |
