8SJR

[3T17] Self-assembling right-handed tensegrity triangle with 17 interjunction base pairs and R3 symmetry

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-21
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.00744
Spacegroup name	H 3
Unit cell lengths	170.214, 170.214, 80.252
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.720 - 5.250
R-factor	0.2117
Rwork	0.209
R-free	0.25010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w6w
RMSD bond length	0.009
RMSD bond angle	1.045
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	85.107	85.107	6.079
High resolution limit [Å]	5.250	13.159	5.253
Rmerge			6.166
Number of reflections	3630	206	205
<I/σ(I)>	6.7	15.1
Completeness [%]	81.0	100
Redundancy	9.7	9.2	10.9
CC(1/2)	0.903	0.886

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	293	100 mM MOPS, 1.25 M magnesium sulfate