8SJP

[3T15] Self-assembling DNA motif with 15 base pairs between junctions and P32 symmetry

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-12
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.00743
Spacegroup name	P 32
Unit cell lengths	100.632, 100.632, 72.676
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.320 - 5.220
R-factor	0.0959
Rwork	0.093
R-free	0.16070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xna
RMSD bond length	0.025
RMSD bond angle	1.984
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	55.815	55.815	5.986
High resolution limit [Å]	5.218	12.999	5.218
Number of reflections	1881	208	213
<I/σ(I)>	9.6	36.9	1
Completeness [%]	84.2
Redundancy	9.6	9
CC(1/2)	0.999	0.999	0.221

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	298	100 mM MOPS, 1.25 M magnesium sulfate