8SJA
Ara H 6 13D9 16A8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-18 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.12713 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 108.466, 112.067, 223.155 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.960 - 2.680 |
| R-factor | 0.1964 |
| Rwork | 0.194 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| Data reduction software | XDS (VERSION Jan 10, 2022 BUILT=20220220) |
| Data scaling software | Aimless (0.7.9) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.960 | 2.780 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rmerge | 0.039 | 0.594 |
| Number of reflections | 76577 | 7559 |
| <I/σ(I)> | 22 | 2.37 |
| Completeness [%] | 99.4 | |
| Redundancy | 4.6 | |
| CC(1/2) | 1.000 | 0.798 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 00 mM Bis Tris pH 5.25, 75 mM Ammonium Acetate, 16% PEG 10,000 |
