8SIL

Lysozyme crystallized in cyclic olefin copolymer-based microfluidic chips

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SLAC LCLS BEAMLINE MFX
Synchrotron site	SLAC LCLS
Beamline	MFX
Temperature [K]	295
Detector technology	PIXEL
Collection date	2022-11-21
Detector	SLAC ePix10k 2M
Wavelength(s)	1.25363
Spacegroup name	P 43 21 2
Unit cell lengths	78.800, 78.800, 38.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.470 - 1.700
R-factor	0.2527
Rwork	0.251
R-free	0.28140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.936
Data reduction software	XDS (Jan 10, 2022)
Data scaling software	pointless (1.12.2)
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.820	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	13913	1353
<I/σ(I)>	4.6	0.5
Completeness [%]	99.8	100
Redundancy	809	435
CC(1/2)	0.948	0.454

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	4.6	277	2 M NaCl with 0.1 M sodium acetate buffer (pH 4.6)