8SHH

Crystal structure of EvdS6 decarboxylase in ligand free state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2021-07-21
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 21 21 21
Unit cell lengths	56.783, 77.489, 146.882
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.740 - 1.930
R-factor	0.1924
Rwork	0.190
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.867
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.950
High resolution limit [Å]	1.920	1.920
Rmerge	0.072
Number of reflections	49037	2387
<I/σ(I)>	27.465
Completeness [%]	99.8
Redundancy	4.7
CC(1/2)	0.994	0.675

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Protein concentration= 9mg/ml, Reservoir solution=0.1M Hepes pH7.0 and 10% w/v [PEG] 6000