8SH4

Crystal structure of the tRNA (m1G37) methyltransferase apoenzyme from Anaplasma phagocytophilum

Replaces:  6UEV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	77
Detector technology	PIXEL
Collection date	2018-06-22
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.9201
Spacegroup name	P 43 21 2
Unit cell lengths	104.730, 104.730, 91.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.400 - 2.900
R-factor	0.2041
Rwork	0.202
R-free	0.25530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3knu
RMSD bond length	0.009
RMSD bond angle	1.130
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX (1.19.1_4122)
Refinement software	PHENIX ((1.19.1_4122: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.400	2.854
High resolution limit [Å]	2.676	2.676
Rmerge	0.093
Number of reflections	11321	566
<I/σ(I)>	16.3
Completeness [%]	88.5
Redundancy	15.5
CC(1/2)	0.999	0.535

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	289	8% PEG 3350, 0.5mL of 0.1M sodium citrate, 10% glycerol, ethylene glycol as cryoprotectant