8SG3

High Affinity nanobodies against GFP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075
Spacegroup name	I 41
Unit cell lengths	108.633, 108.633, 198.744
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.190 - 3.110
R-factor	0.2762
Rwork	0.273
R-free	0.30350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.812
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.170
High resolution limit [Å]	3.110	8.130	3.110
Rmerge	0.212	0.098	0.833
Total number of observations	72098
Number of reflections	19752	926	1067
<I/σ(I)>	5
Completeness [%]	84.6	77.5	92.6
Redundancy	3.7	2.9	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.2 M sodium potassium phosphate pH 6.2, 2.5 M sodium chloride