8SG3
High Affinity nanobodies against GFP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075 |
| Spacegroup name | I 41 |
| Unit cell lengths | 108.633, 108.633, 198.744 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.190 - 3.110 |
| R-factor | 0.2762 |
| Rwork | 0.273 |
| R-free | 0.30350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.812 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.170 |
| High resolution limit [Å] | 3.110 | 8.130 | 3.110 |
| Rmerge | 0.212 | 0.098 | 0.833 |
| Total number of observations | 72098 | ||
| Number of reflections | 19752 | 926 | 1067 |
| <I/σ(I)> | 5 | ||
| Completeness [%] | 84.6 | 77.5 | 92.6 |
| Redundancy | 3.7 | 2.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2 M sodium potassium phosphate pH 6.2, 2.5 M sodium chloride |
