8SFV
High affinity nanobodies to GFP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0750 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 111.251, 111.251, 193.802 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.250 - 1.830 |
| R-factor | 0.1835 |
| Rwork | 0.183 |
| R-free | 0.20590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.150 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.860 |
| High resolution limit [Å] | 1.830 | 4.880 | 1.830 |
| Rmerge | 0.080 | 0.053 | 0.880 |
| Total number of observations | 805788 | ||
| Number of reflections | 107447 | 5391 | 5355 |
| <I/σ(I)> | 9.8 | ||
| Completeness [%] | 99.6 | 99.5 | 100 |
| Redundancy | 7.5 | 7.4 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2 M lithium sulphate, 0.1 M tris pH 7.0, 1 M potassium sodium tartrate |
