8SFG
Crystal Structure of the Open Unbound Catalytically Inactive Makes Caterpillars Floppy-like (MCF) Effector from Vibrio vulnificus CMCP6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-10-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.381, 160.661, 71.793 |
| Unit cell angles | 90.00, 101.28, 90.00 |
Refinement procedure
| Resolution | 29.870 - 2.800 |
| R-factor | 0.1914 |
| Rwork | 0.189 |
| R-free | 0.22960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.203 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.110 | 0.751 |
| Rmeas | 0.125 | 0.857 |
| Rpim | 0.059 | 0.411 |
| Number of reflections | 32763 | 1579 |
| <I/σ(I)> | 13.3 | 1.9 |
| Completeness [%] | 97.7 | 94.7 |
| Redundancy | 4.4 | 4.3 |
| CC(1/2) | 0.627 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 292 | Protein: 8.4 mg/ml, 0.5 Sodium chloride, 0.01M Tris pH 8.3; Screen: AmSO4 Suite (B3), 0.2M Cesium sulfate, 2.2M Ammonium sulfate. Cryo: 1.8M Ammonim sulfate, 25% Succrose |
