8SCU
Bst DNA polymerase I Large Fragment wildtype D598A with 3'-amino primer, dGTP, and calcium time-resolved 48h (Product State)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 99 |
Detector technology | PIXEL |
Collection date | 2021-07-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.033175 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 94.082, 109.164, 150.591 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.040 - 2.380 |
R-factor | 0.1896 |
Rwork | 0.187 |
R-free | 0.23910 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.948 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.420 |
High resolution limit [Å] | 2.380 | 2.380 |
Rmerge | 0.107 | 0.497 |
Rmeas | 0.121 | 0.559 |
Rpim | 0.054 | 0.253 |
Number of reflections | 61520 | 3062 |
<I/σ(I)> | 11.8 | 2.8 |
Completeness [%] | 98.7 | 99.8 |
Redundancy | 4.7 | 4.7 |
CC(1/2) | 0.988 | 0.799 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2M ammonium sulphate, 0.1M MES pH 5.3-6.0, 20 mM CaCl2, 5% MPD |