8SCP
Bst DNA polymerase I Large Fragment wildtype D598A with 3'-amino primer, dGTP, and calcium time-resolved 1h
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 99 |
Detector technology | PIXEL |
Collection date | 2021-07-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.033175 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 93.864, 108.751, 149.986 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.860 - 2.080 |
R-factor | 0.1915 |
Rwork | 0.189 |
R-free | 0.23790 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.922 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.120 |
High resolution limit [Å] | 2.080 | 2.080 |
Rmerge | 0.079 | 0.471 |
Rmeas | 0.086 | 0.512 |
Rpim | 0.035 | 0.199 |
Number of reflections | 90185 | 4558 |
<I/σ(I)> | 21.7 | 4.2 |
Completeness [%] | 97.0 | 100 |
Redundancy | 6 | 6.4 |
CC(1/2) | 0.992 | 0.899 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2M ammonium sulphate, 0.1M MES pH 5.3-6.0, 20 mM CaCl2, 5% MPD |