8SCO
Bst DNA polymerase I Large Fragment wildtype D598A with 3'-amino primer, dGTP, and calcium time-resolved 0h (Ground State)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 99 |
| Detector technology | PIXEL |
| Collection date | 2021-02-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033175 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 94.051, 109.038, 150.945 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.520 - 1.920 |
| R-factor | 0.1913 |
| Rwork | 0.189 |
| R-free | 0.23370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.905 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.055 | 0.389 |
| Rmeas | 0.060 | 0.431 |
| Rpim | 0.024 | 0.181 |
| Number of reflections | 111554 | 4420 |
| <I/σ(I)> | 29.9 | 4.1 |
| Completeness [%] | 93.6 | 75.6 |
| Redundancy | 6.3 | 5.4 |
| CC(1/2) | 0.997 | 0.875 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2M ammonium sulphate, 0.1M MES pH 5.3-6.0, 20 mM CaCl2, 5% MPD |
