8SBK
Structure of HLA-A*24:02 in complex with peptide, LYLPVRVLI (ATG2A).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-02 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.920 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 155.668, 155.668, 155.668 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 110.070 - 1.800 |
| R-factor | 0.1937 |
| Rwork | 0.192 |
| R-free | 0.21890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.920 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.4 (21-NOV-2022)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 110.070 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.220 | 2.925 |
| Rmeas | 0.228 | 3.023 |
| Rpim | 0.058 | 0.762 |
| Number of reflections | 57961 | 3418 |
| <I/σ(I)> | 10.7 | 1.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.6 | 7.8 |
| CC(1/2) | 0.998 | 0.426 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 2 M Ammonium sulfate, 0.1 M Sodium cacodylate pH 6.5 |
