8SBK

Structure of HLA-A*24:02 in complex with peptide, LYLPVRVLI (ATG2A).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-02
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920
Spacegroup name	I 2 3
Unit cell lengths	155.668, 155.668, 155.668
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	110.070 - 1.800
R-factor	0.1937
Rwork	0.192
R-free	0.21890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.920
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	BUSTER (2.10.4 (21-NOV-2022))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	110.070	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.220	2.925
Rmeas	0.228	3.023
Rpim	0.058	0.762
Number of reflections	57961	3418
<I/σ(I)>	10.7	1.2
Completeness [%]	100.0	100
Redundancy	7.6	7.8
CC(1/2)	0.998	0.426

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	294	2 M Ammonium sulfate, 0.1 M Sodium cacodylate pH 6.5