8S9O

DNA cytosine-N4 methyltransferase (residues 61-324) from the Bdelloid rotifer Adineta vaga - P1 crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-01
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9201
Spacegroup name	P 1
Unit cell lengths	49.646, 53.301, 54.376
Unit cell angles	97.04, 104.34, 98.20

Refinement procedure

Resolution	26.020 - 1.940
R-factor	0.208
Rwork	0.206
R-free	0.24840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.588
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.960	1.970
High resolution limit [Å]	1.940	1.940
Rpim	0.113
Number of reflections	38198	1866
<I/σ(I)>	3.6	0.6
Completeness [%]	97.1
Redundancy	2.2
CC(1/2)	0.987	0.323

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.2 M Sodium thiocyanate, 20 % PEG 3350