8S9O
DNA cytosine-N4 methyltransferase (residues 61-324) from the Bdelloid rotifer Adineta vaga - P1 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-01 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9201 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.646, 53.301, 54.376 |
| Unit cell angles | 97.04, 104.34, 98.20 |
Refinement procedure
| Resolution | 26.020 - 1.940 |
| R-factor | 0.208 |
| Rwork | 0.206 |
| R-free | 0.24840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.588 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.960 | 1.970 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rpim | 0.113 | |
| Number of reflections | 38198 | 1866 |
| <I/σ(I)> | 3.6 | 0.6 |
| Completeness [%] | 97.1 | |
| Redundancy | 2.2 | |
| CC(1/2) | 0.987 | 0.323 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.2 M Sodium thiocyanate, 20 % PEG 3350 |
