8S9N

DNA cytosine-N4 methyltransferase (residues 61-324) from the Bdelloid rotifer Adineta vaga - C2 crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-01
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9201
Spacegroup name	C 1 2 1
Unit cell lengths	71.426, 82.285, 49.773
Unit cell angles	90.00, 106.57, 90.00

Refinement procedure

Resolution	34.230 - 1.890
R-factor	0.2022
Rwork	0.201
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.655
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.630	1.930
High resolution limit [Å]	1.890	1.890
Rmerge	0.092
Rpim	0.050
Number of reflections	21990	1082
<I/σ(I)>	6.8	0.6
Completeness [%]	99.7	99.4
Redundancy	4.3	4.5
CC(1/2)	0.996	0.435

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	0.2 M Sodium chloride, 0.1 M Bis-Tris pH 6.5, 25% PEG 3350