8S95
Crystal Structure of Poliovirus (type 1 Mahoney) cloverleaf RNA with tRNA scaffold
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ DW |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2021-06-03 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.542 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 150.021, 28.015, 111.267 |
| Unit cell angles | 90.00, 98.04, 90.00 |
Refinement procedure
| Resolution | 47.540 - 3.100 |
| R-factor | 0.2756 |
| Rwork | 0.275 |
| R-free | 0.28740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.587 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.540 | 3.150 |
| High resolution limit [Å] | 2.990 | 3.100 |
| Rpim | 0.127 | 0.635 |
| Number of reflections | 8747 | 2838 |
| <I/σ(I)> | 6.5 | |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 5.4 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 292 | 47% polypropylene glycol P 400, 100mM Imidazole pH 7.4. 1uL of mother liquor mixed with 1 uL RNA (4mg/mL), and then before sealing well, 10%(v/v) methanol was added to mother liquor, not drop |
