8S6S

Oxidoreductase from Phytophthora sojae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	46.267, 73.129, 116.418
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.925 - 1.789
Rwork	0.161
R-free	0.20230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.560
Data reduction software	AutoProcess
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.925	1.820
High resolution limit [Å]	1.789	1.789
Number of reflections	38093	1838
<I/σ(I)>	16.3
Completeness [%]	100.0
Redundancy	13.3
CC(1/2)	0.998	0.829

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.2 M Lithium sulfate, 0.1 M Tris pH 8.5, 30% w/v PEG 4000