8S6G
Carbohydrate active oxidoreductase from Alternaria alternata
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.893, 69.541, 62.700 |
| Unit cell angles | 90.00, 93.97, 90.00 |
Refinement procedure
| Resolution | 30.300 - 1.210 |
| R-factor | 0.1634 |
| Rwork | 0.162 |
| R-free | 0.18420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.199 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.560 | 1.230 |
| High resolution limit [Å] | 1.208 | 1.208 |
| Number of reflections | 159342 | 8198 |
| <I/σ(I)> | 14.7 | |
| Completeness [%] | 98.3 | |
| Redundancy | 6.6 | |
| CC(1/2) | 0.999 | 0.754 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.1M sodium HEPES pH 7.5, 20% w/v PEG 10K |
