8S6E

Monoclonal antibody MenW targeting serogroup W of Neisseria meningitidis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	43.870, 69.160, 128.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.270 - 1.950
R-factor	0.201
Rwork	0.201
R-free	0.22310
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	MAIN (2023)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	1.950	1.950
Number of reflections	25519	3677
<I/σ(I)>	16.46
Completeness [%]	86.7
Redundancy	7.74
CC(1/2)	0.998	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0,2 M sodium acetate 0,1 M TRIS, pH 8,5 30% w/v PEG 4000