8S2U
SSX structure of Lysozyme grown in batch conditions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2023-01-23 |
Detector | PSI JUNGFRAU 4M |
Wavelength(s) | 1.07 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.000, 79.000, 37.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.924 - 1.800 |
Rwork | 0.171 |
R-free | 0.23070 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.551 |
Data reduction software | CrystFEL (0.10.2) |
Data scaling software | CrystFEL (0.10.2) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 11636 | 669 |
<I/σ(I)> | 13.5 | 0.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 438 | 47.67 |
CC(1/2) | 0.990 | 0.490 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 3 | 294 | Protein dissolved in 20 mM sodium acetate pH 4.6 Protein and precipitant mixed in a 1:4 ratio Precipitant solution: 6% PEG 6000 (w/v), 3.4M NaCl and 1M sodium acetate pH 3.0 |