8S1V

Crystal structure of full-lenght Renilla reniformis green fluorescent protein (GFP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-13
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	82.426, 82.426, 82.222
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.862 - 1.943
R-factor	0.2062
Rwork	0.204
R-free	0.24920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.599
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.550	1.990
High resolution limit [Å]	1.940	1.940
Number of reflections	21444	1315
<I/σ(I)>	28.1
Completeness [%]	99.6
Redundancy	10.9
CC(1/2)	1.000	0.646

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	MES buffer, PEG4000