8RZV

Structure of UP1 S4ES6E phosphomimetic mutant in complex with human telomeric repeat DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	51.120, 51.120, 173.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.030 - 1.510
R-factor	0.1974
Rwork	0.196
R-free	0.21960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.950
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.030	1.560
High resolution limit [Å]	1.510	1.510
Rmeas	0.084
Number of reflections	36927	3543
<I/σ(I)>	22.9	1.3
Completeness [%]	99.2	98
Redundancy	2	2
CC(1/2)	1.000	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289	0.1 M Tris, pH 8.50, 1.7 M Ammonium Phosphate Dibasic, 15 % (v/v) glycerol