8RZH
ZgGH129 from Zobellia galactanivorans in complex with the inhibitor AD-DGJ (3,6-anhydro-D-1-deoxygalactonojirimycin).
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-22 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9786 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 219.204, 106.492, 165.341 |
Unit cell angles | 90.00, 113.80, 90.00 |
Refinement procedure
Resolution | 41.320 - 1.830 |
R-factor | 0.1374 |
Rwork | 0.136 |
R-free | 0.16240 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.170 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.16) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.420 | 1.930 |
High resolution limit [Å] | 1.830 | 1.830 |
Rmeas | 0.064 | 0.433 |
Number of reflections | 303236 | 44194 |
<I/σ(I)> | 10.9 | 3 |
Completeness [%] | 99.3 | 99.3 |
Redundancy | 2.9 | 2.9 |
CC(1/2) | 0.998 | 0.848 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2:1 ratio 0.14M Na/K-tartrate, 14% PEG 3350 1.63 mg/ml |