8RZH
ZgGH129 from Zobellia galactanivorans in complex with the inhibitor AD-DGJ (3,6-anhydro-D-1-deoxygalactonojirimycin).
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-22 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 219.204, 106.492, 165.341 |
| Unit cell angles | 90.00, 113.80, 90.00 |
Refinement procedure
| Resolution | 41.320 - 1.830 |
| R-factor | 0.1374 |
| Rwork | 0.136 |
| R-free | 0.16240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.170 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.16) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.420 | 1.930 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmeas | 0.064 | 0.433 |
| Number of reflections | 303236 | 44194 |
| <I/σ(I)> | 10.9 | 3 |
| Completeness [%] | 99.3 | 99.3 |
| Redundancy | 2.9 | 2.9 |
| CC(1/2) | 0.998 | 0.848 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2:1 ratio 0.14M Na/K-tartrate, 14% PEG 3350 1.63 mg/ml |
