8RZA
Ribonuclease W
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 120.620, 120.620, 93.840 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.640 - 2.100 |
| R-factor | 0.2136 |
| Rwork | 0.212 |
| R-free | 0.24380 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.949 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.640 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 46329 | 4588 |
| <I/σ(I)> | 17.8 | 1.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 20 | |
| CC(1/2) | 0.900 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | trisodium citrate, PEG 3350 |
