8RZ2

PfRH5 bound to monoclonal antibody R5.034

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9786
Spacegroup name	P 21 21 21
Unit cell lengths	82.754, 82.993, 118.746
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	68.030 - 2.400
R-factor	0.2574
Rwork	0.255
R-free	0.30460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.870
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.4 (26-JUL-2023))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.990	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	32741	3402
<I/σ(I)>	15.2
Completeness [%]	100.0
Redundancy	12.8
CC(1/2)	0.999	0.911

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2 M ammonium chloride, 20% w/v PEG 3350