8RZ1
Synthetic immunogen RH5-34EM bound to monoclonal antibody R5.034
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-01-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 61 |
| Unit cell lengths | 111.830, 111.830, 91.570 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.910 - 1.750 |
| R-factor | 0.1986 |
| Rwork | 0.198 |
| R-free | 0.21840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.950 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4 (26-JUL-2023)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.920 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 65543 | 3252 |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 10 | |
| CC(1/2) | 0.998 | 0.598 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M Buffer System 1 pH 6.5, 0.06 M Divalents, 30% v/v Precipitant Mix 1 |
