8RYS

Human IL-1beta, unliganded

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.999980
Spacegroup name	P 43
Unit cell lengths	54.784, 54.784, 76.173
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.390 - 1.160
R-factor	0.2068
Rwork	0.206
R-free	0.22080
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.014
RMSD bond angle	1.340
Data reduction software	autoPROC (1.1.7)
Data scaling software	Aimless (0.7.9)
Phasing software	BUSTER (2.11.8)
Refinement software	BUSTER (2.11.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.476	1.180
High resolution limit [Å]	1.160	1.160
Rmerge	0.030	1.032
Rmeas	0.033	1.294
Rpim	0.013	0.762
Number of reflections	71960	2033
<I/σ(I)>	23.4	0.7
Completeness [%]	92.7	52.8
Redundancy	6	2.5
CC(1/2)	0.999	0.363

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1M TRIS pH 8.5, 2.4M ammonium phosphate