8RXA

ACDC domain of AP2-O5 from Plasmodium falciparum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	77
Detector technology	PIXEL
Collection date	2022-06-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980112
Spacegroup name	P 21 21 2
Unit cell lengths	58.930, 108.930, 34.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.998 - 1.751
R-factor	0.2118
Rwork	0.210
R-free	0.24860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.304
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	5.210	1.860
High resolution limit [Å]	1.750	3.700	1.750
Rmerge	0.056	0.038	0.937
Rmeas	0.059	0.039	0.974
Number of reflections	22718	1612	3510
<I/σ(I)>	22.24
Completeness [%]	99.5
Redundancy	13
CC(1/2)	0.999	0.999	0.941

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	30% PEG400, 0.1 M MES pH 6.5, 0.1 M Sodium acetate