8RX2
Domains 1 and 2 of Sap S-layer protein from Bacillus anthracis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-18 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.444, 128.612, 55.128 |
| Unit cell angles | 90.00, 95.76, 90.00 |
Refinement procedure
| Resolution | 41.730 - 2.010 |
| R-factor | 0.2028 |
| Rwork | 0.201 |
| R-free | 0.23690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.896 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20_4459: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.730 | 2.086 |
| High resolution limit [Å] | 2.010 | 2.014 |
| Rpim | 0.033 | 0.268 |
| Number of reflections | 44265 | 4388 |
| <I/σ(I)> | 2.4 | |
| Completeness [%] | 99.9 | |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.09 M Halogens, 0.1 M Buffer System 1 6.5 (pH) and 37.5% v/v Precipitant Mix 41 |
