8RWU

ACDC domain of the AP2-I transcription factor from Plasmodium vivax

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980118
Spacegroup name	P 41 21 2
Unit cell lengths	78.490, 78.490, 123.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.620 - 3.150
R-factor	0.2286
Rwork	0.226
R-free	0.27370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.727
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	9.300	3.340
High resolution limit [Å]	3.150	6.630	3.150
Rmerge	0.115	0.045	3.024
Rmeas	0.118	0.046	3.084
Number of reflections	7133	520	1106
<I/σ(I)>	21.53		1.18
Completeness [%]	99.7
Redundancy	25
CC(1/2)	1.000	1.000	0.495

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	30% PEG 400 , 0.1 M Mes pH 6.5, 0.1 M Sodium acetate