8RW6

Human Brain and muscle ARNT-like 1 (BMAL1_HUMAN) PAS-B domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-07
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	30.236, 37.348, 76.116
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.060 - 1.830
R-factor	0.2044
Rwork	0.202
R-free	0.24650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.817
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.060	1.564
High resolution limit [Å]	1.510	1.510
Number of reflections	14123	1365
<I/σ(I)>	7.22
Completeness [%]	98.0
Redundancy	13.1
CC(1/2)	0.997	0.729

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	20 % PEG3350, 0.15 M sodium formate