8RVX

Cph1 phytochrome PAS-GAF-PHY Y176H mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.918400
Spacegroup name	P 43 21 2
Unit cell lengths	135.873, 135.873, 355.644
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.651 - 3.696
Rwork	0.266
R-free	0.30680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.338
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.651	3.791
High resolution limit [Å]	3.696	3.696
Rmerge	0.800	3.702
Rpim	0.157	0.743
Number of reflections	36339	4242
<I/σ(I)>	6.3	1.2
Completeness [%]	99.3	97.2
Redundancy	25.8	24.8
CC(1/2)	0.982	0.398

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	293	0.3 M ammonium formate 0.1 M HEPES pH 6.9 18% (w/v) Sokalan CP7