8RVF
CRYSTAL STRUCTURE OF HUMAN MONOGLYCERIDE LIPASE IN COMPLEX WITH COMPOUND 5
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-17 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 90.446, 127.312, 62.845 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.660 - 1.430 |
| R-factor | 0.1948 |
| Rwork | 0.194 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.180 | 1.530 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.078 | 1.000 |
| Number of reflections | 67210 | 12202 |
| <I/σ(I)> | 8.34 | |
| Completeness [%] | 100.0 | |
| Redundancy | 7.47 | |
| CC(1/2) | 0.990 | 0.661 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M MES pH 6.5, 11% PEG MME5K, 12% isopropanol |
