8RVC
Crystal structure of alpha keto acid C-methyl-transferases MrsA bound to ketoarginine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-01 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 148.450, 65.873, 75.041 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.510 - 1.969 |
| R-factor | 0.1959 |
| Rwork | 0.195 |
| R-free | 0.21970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.910 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.510 | 2.020 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.148 | 0.978 |
| Rmeas | 0.154 | 1.033 |
| Rpim | 0.043 | 0.318 |
| Number of reflections | 51937 | 2612 |
| <I/σ(I)> | 14 | 2.3 |
| Completeness [%] | 98.0 | 71.7 |
| Redundancy | 12.6 | 9.1 |
| CC(1/2) | 0.998 | 0.755 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 100mM HEPES, 300mM potassium chloride, 28% PEG 3350 |
