8RVC
Crystal structure of alpha keto acid C-methyl-transferases MrsA bound to ketoarginine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-01 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 148.450, 65.873, 75.041 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.510 - 1.969 |
R-factor | 0.1959 |
Rwork | 0.195 |
R-free | 0.21970 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.910 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.510 | 2.020 |
High resolution limit [Å] | 1.970 | 1.970 |
Rmerge | 0.148 | 0.978 |
Rmeas | 0.154 | 1.033 |
Rpim | 0.043 | 0.318 |
Number of reflections | 51937 | 2612 |
<I/σ(I)> | 14 | 2.3 |
Completeness [%] | 98.0 | 71.7 |
Redundancy | 12.6 | 9.1 |
CC(1/2) | 0.998 | 0.755 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 100mM HEPES, 300mM potassium chloride, 28% PEG 3350 |