8RUS
Hen egg-white lysozyme (HEWL) structure from EuXFEL FXE, multi-hit Droplet-on-Demand (DoD) injection, 9.3 keV photon energy, space group P432121
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | EUROPEAN XFEL BEAMLINE FXE |
Synchrotron site | European XFEL |
Beamline | FXE |
Temperature [K] | 298 |
Detector technology | PIXEL |
Collection date | 2022-11-02 |
Detector | STFC Large Pixel Detector |
Wavelength(s) | 1.333 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.470, 79.470, 38.320 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.730 - 1.380 |
R-factor | 0.1535 |
Rwork | 0.148 |
R-free | 0.19980 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 0.948 |
Data reduction software | CrystFEL |
Data scaling software | CrystFEL |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.730 | 1.400 |
High resolution limit [Å] | 1.380 | 1.380 |
Number of reflections | 12383860 | 107809 |
<I/σ(I)> | 6.77 | 0.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 479 | 43 |
CC(1/2) | 0.990 | 0.010 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 3.5 | 298 | 1:2 lysozyme (100 mg mL-1 in 50 mM NaOAc (pH 3.5)) and crystallization solution (0.1 M NaOAc (pH 3.5), 5% PEG6000 (v/v), 3.2 M NaCl) at 298K. |