8RUL
Structure of Oceanobacillus iheyensis group II intron in the presence of Li+ and Mg2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-12 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.96 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 89.169, 95.487, 224.306 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.180 - 3.620 |
| R-factor | 0.16396 |
| Rwork | 0.160 |
| R-free | 0.23445 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 2.077 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.180 | 3.749 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Number of reflections | 22529 | 2210 |
| <I/σ(I)> | 13.08 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.3 | |
| CC(1/2) | 0.999 | 0.675 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 303.15 | 100 mM Mg-Acetate, 150 mM LiCl, 50 mM Na-HEPES pH 7.0, 5%PEG 8000 |
