8RTP
Bilirubin oxidase from Myrothecium verrucaria in complex with NAD+
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 134.727, 202.441, 224.762 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.992 - 2.100 |
| R-factor | 0.1686 |
| Rwork | 0.169 |
| R-free | 0.21520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.684 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.992 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.115 | 0.692 |
| Rmeas | 0.128 | 0.782 |
| Rpim | 0.055 | 0.354 |
| Number of reflections | 87859 | 4073 |
| <I/σ(I)> | 10.2 | 2 |
| Completeness [%] | 98.9 | 90.9 |
| Redundancy | 5.4 | 4.5 |
| CC(1/2) | 0.993 | 0.723 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.1 | 291.15 | 14% (w/v) PEG 3350, 0.1 M succinic acid, pH 3.1, protein concentration 25 mg/ml. |
