8RTE

Rap from bacteriophage Phi105 with peptide ERPVGT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-03
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	65.300, 80.307, 91.707
Unit cell angles	90.00, 100.68, 90.00

Refinement procedure

Resolution	90.120 - 2.400
R-factor	0.2205
Rwork	0.219
R-free	0.25540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.645
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.120	2.486
High resolution limit [Å]	2.400	2.400
Number of reflections	36368	3628
<I/σ(I)>	5.73
Completeness [%]	99.4
Redundancy	6.7
CC(1/2)	0.978	0.978

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	294	20% glycerol ethoxylate, 10% tetrahydrofuran and 0.1M tris-HCl pH8