8RT3

Hen Egg White Lysozyme Crystallized with Bioassembler in Space Microgravity Conditions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	P 43 21 2
Unit cell lengths	77.210, 77.210, 39.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.450 - 1.090
R-factor	0.123
Rwork	0.122
R-free	0.14170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.588
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.850	1.120
High resolution limit [Å]	1.090	1.090
Number of reflections	91860	5759
<I/σ(I)>	15.3
Completeness [%]	98.1
Redundancy	5.4
CC(1/2)	0.996	0.623

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.8	294	SODIUM ACETATE 0.1M AT PH 4.8, NACL 13%, Gd(HP-D03A) 250mM, 294K