8RT0
BTV-15 VP5 pH 6.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2015-12-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96864 |
| Spacegroup name | H 3 |
| Unit cell lengths | 76.870, 76.870, 163.110 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.570 - 2.190 |
| R-factor | 0.20069 |
| Rwork | 0.199 |
| R-free | 0.22908 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.868 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.800 | 2.250 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.070 | |
| Number of reflections | 18479 | 1322 |
| <I/σ(I)> | 13.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 9.9 | |
| CC(1/2) | 0.650 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 1M Sodium Chloride, 0.1M Tris, pH 6.0 |
