8RSV
Rap from bacteriophage Phi3T in presence of pheromone RRGHTAS
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.490, 92.332, 100.670 |
| Unit cell angles | 90.00, 106.23, 90.00 |
Refinement procedure
| Resolution | 58.080 - 2.190 |
| R-factor | 0.2112 |
| Rwork | 0.210 |
| R-free | 0.23790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.975 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.080 | 2.230 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Number of reflections | 53486 | 5389 |
| <I/σ(I)> | 24.44 | |
| Completeness [%] | 97.6 | |
| Redundancy | 17.8 | |
| CC(1/2) | 0.838 | 0.630 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 294 | 25% glycerol ethoxylate, 0.2M NH4Cl |
