8RSQ

Crystal structure of marine actinobacteria clade rhodopsin (MAR) - human GTPase Arf1 (L8K,Q71L) chimera; Ground state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	293
Detector technology	PIXEL
Collection date	2021-07-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9762
Spacegroup name	C 1 2 1
Unit cell lengths	132.856, 39.718, 94.984
Unit cell angles	90.00, 124.63, 90.00

Refinement procedure

Resolution	37.330 - 2.300
R-factor	0.2167
Rwork	0.211
R-free	0.26340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.455
Data reduction software	XDS (20210323)
Data scaling software	STARANISO (2.3.77)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.330	37.330	2.410
High resolution limit [Å]	2.300	6.580	2.300
Rpim	0.052	0.024	0.409
Number of reflections	15145	757	759
<I/σ(I)>	8	21.2	1.6
Completeness [%]	88.9	89.2	69.7
Redundancy	2.3	2.1	1.6
CC(1/2)	0.998	0.998	0.702

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	7	293	2.0 M Ammonium Phosphate buffer