8RQY
MakC and MakD are two proteins associated with a tripartite toxin of Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-14 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 46.998, 98.008, 54.528 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.400 - 2.045 |
| Rwork | 0.183 |
| R-free | 0.19970 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.989 |
| Data reduction software | autoPROC (1.1.7) |
| Data scaling software | autoPROC (1.1.7) |
| Phasing software | CRANK2 (2.0.329) |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.400 | 2.080 |
| High resolution limit [Å] | 2.040 | 2.045 |
| Rmerge | 0.177 | 1.134 |
| Rmeas | 0.192 | |
| Rpim | 0.073 | 0.451 |
| Number of reflections | 8027 | 395 |
| <I/σ(I)> | 8.5 | 2 |
| Completeness [%] | 97.1 | 100 |
| Redundancy | 6.8 | 7.3 |
| CC(1/2) | 0.994 | 0.760 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.2 M potassium sodium tartarate, 0.1 M sodium citrate, pH 5.6 and 2M Ammonium sulfate |
