8RQI

Structure of Rhizobium NopD with ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 43
Unit cell lengths	86.496, 86.496, 46.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.250 - 1.940
R-factor	0.173
Rwork	0.172
R-free	0.19940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Alphafold-2
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.250	2.043
High resolution limit [Å]	1.940	1.942
Number of reflections	25728	1141
<I/σ(I)>	13.3
Completeness [%]	94.2
Redundancy	6.8
CC(1/2)	0.997	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M imidazole 8.0 and 10% PEG8000