8RQH

Crystal Structure of the flavoprotein monooxygenase TrlE from Streptomyces cyaneofuscatus Soc7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00003
Spacegroup name	P 31 2 1
Unit cell lengths	112.900, 112.900, 148.570
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.572 - 2.500
Rwork	0.191
R-free	0.23850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.365
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.589
High resolution limit [Å]	2.500	2.500
Number of reflections	38458	3789
<I/σ(I)>	14.02
Completeness [%]	99.9
Redundancy	20.6
CC(1/2)	0.999	0.554

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293.15	0.1 M BIS-TRIS propane pH 8.5 0.25 M Na2SO4 15% w/v PEG3350