8RQH
Crystal Structure of the flavoprotein monooxygenase TrlE from Streptomyces cyaneofuscatus Soc7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-24 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00003 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 112.900, 112.900, 148.570 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.572 - 2.500 |
Rwork | 0.191 |
R-free | 0.23850 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.365 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.589 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 38458 | 3789 |
<I/σ(I)> | 14.02 | |
Completeness [%] | 99.9 | |
Redundancy | 20.6 | |
CC(1/2) | 0.999 | 0.554 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.15 | 0.1 M BIS-TRIS propane pH 8.5 0.25 M Na2SO4 15% w/v PEG3350 |