8RQD
Crystal structure of human DNPH1 mutant-Y24F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-08 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.6199 |
| Spacegroup name | H 3 |
| Unit cell lengths | 139.907, 139.907, 33.313 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.420 - 2.140 |
| R-factor | 0.18191 |
| Rwork | 0.179 |
| R-free | 0.23697 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.618 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.030 | 2.200 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.186 | 1.028 |
| Rmeas | 0.206 | |
| Rpim | 0.088 | 0.493 |
| Number of reflections | 13475 | 1129 |
| <I/σ(I)> | 7.7 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.4 | 10.3 |
| CC(1/2) | 0.996 | 0.885 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.15 | 0.2 M ammonium sulfate, 0.1 M MES pH 6.5 30 % PEG 5000 MME |
