8RQ1
Crystal structure of CRBN-midi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 52.031, 96.405, 148.086 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 74.040 - 3.110 |
| R-factor | 0.2684 |
| Rwork | 0.267 |
| R-free | 0.29970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.605 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.040 | 3.160 |
| High resolution limit [Å] | 3.110 | 3.110 |
| Rmerge | 0.410 | 6.797 |
| Number of reflections | 6975 | 688 |
| <I/σ(I)> | 5 | 0.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.7 | 11.6 |
| CC(1/2) | 0.980 | 0.370 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2 M sodium citrate, 20% (w/v) PEG 3350, and 0.1 M BIS-TRIS propane pH 6.5 |
